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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
29/07/2022 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
DOI : |
10.3389/fvets.2022.889157 |
Idioma : |
Inglés |
Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
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Marc : |
LEADER 03260naa a2200325 a 4500 001 1063470 005 2022-09-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fvets.2022.889157$2DOI 100 1 $aGIANNITTI, F. 245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. 520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. 653 $aABORTION 653 $aDAIRY CATTLE 653 $aGENETIC DIVERSITY 653 $aMolecular epidemiology 653 $aMultilocus microsatellite typin 653 $aNeospora caninum 653 $aPATHOLOGY 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aREPRODUCTIVE DISEASES 700 1 $aARÁOZ, V. 700 1 $aSILVEIRA, C.S. 700 1 $aFRANCIA, M.E. 700 1 $aROBELLO, C. 700 1 $aCABRERA, A. 773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
30/09/2014 |
Actualizado : |
25/09/2019 |
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
Autor : |
RESTAINO, E.; FASSIO, A.; COZZOLINO, D. |
Afiliación : |
ERNESTO ANGEL RESTAINO GALUP, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALBERTO SANTIAGO FASSIO ARAUJO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Discrimination of meat patés according to the animal species by means of near infrared spectroscopy and chemometrics. [Discriminación de muestras de paté de carne según tipo de especie mediante el uso de la espectroscopia en el infrarrojo cercano y la quimiometria.] |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
CYTA - Journal of Food, v. 9, n. 3, p. 210-213, 2011. |
ISSN : |
1947-6337 |
DOI : |
10.1080/19476337.2010.512396 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Commercial meat paté samples, comprised of 100% pork (n = 7), 100% beef (n = 5) meat, and binary mixtures (beef and pork, w/w) (n = 18) were used. Fresh samples were analysed in a scanning spectrophotometer NIRSystems 6500 in reflectance mode (1100?2500 nm). Principal component analysis (PCA) and stepwise linear discriminant analysis (SLDA) were used to classify samples according to the animal species based on their near infrared reflectance (NIR) spectra. Full cross validation was used as validation method when classification models were developed. Both beef and pork paté samples were classified correctly (100%) while binary mixture samples only achieved 72% of correct classification using SLDA technique. The results demonstrated the usefulness of NIR spectra combined with chemometrics as an objective and rapid method to classify paté samples according to meat type. Nevertheless, NIR spectroscopic methods might provide initial screening in the food chain and enable more costly methods to be used more efficiently.
RESUMEN.
Se utilizaron muestras de patés de carne comerciales, compuestos de 100% carne de cerdo (n = 7), 100% carne de buey (n = 5) y mezclas binarias (buey y cerdo) (n = 18). Las muestras frescas se analizaron en un espectrofotómetro de escaneo NIRSystems 6500 en modo reflectante (1100?2500 nm). El análisis de componente principal (PCA) y el análisis discriminante lineal progresivo (SLDA) se usaron para clasificar muestras de según las especie animal basándose en sus espectros NIR. La validación cruzada completa se utilizó como método de validación cuando se desarrollaron métodos de clasificación. Las muestras de ambos patés, de buey y cerdo, se clasificaron correctamente (100%), mientras las muestras de mezcla binaria sólo alcanzaron un 72% de clasificación correcta usando la técnica SLDA. Los resultados mostraron la utilidad del espectro NIR combinado con quimometria como un objetivo y método rápido para clasificar muestras de paté según el tipo de carne. Sin embargo, los métodos espectroscópicos NIR podrían proveer una revisión inicial de la cadena de comida y permitir que métodos costosos fuesen usados más eficientemente. MenosABSTRACT.
Commercial meat paté samples, comprised of 100% pork (n = 7), 100% beef (n = 5) meat, and binary mixtures (beef and pork, w/w) (n = 18) were used. Fresh samples were analysed in a scanning spectrophotometer NIRSystems 6500 in reflectance mode (1100?2500 nm). Principal component analysis (PCA) and stepwise linear discriminant analysis (SLDA) were used to classify samples according to the animal species based on their near infrared reflectance (NIR) spectra. Full cross validation was used as validation method when classification models were developed. Both beef and pork paté samples were classified correctly (100%) while binary mixture samples only achieved 72% of correct classification using SLDA technique. The results demonstrated the usefulness of NIR spectra combined with chemometrics as an objective and rapid method to classify paté samples according to meat type. Nevertheless, NIR spectroscopic methods might provide initial screening in the food chain and enable more costly methods to be used more efficiently.
RESUMEN.
Se utilizaron muestras de patés de carne comerciales, compuestos de 100% carne de cerdo (n = 7), 100% carne de buey (n = 5) y mezclas binarias (buey y cerdo) (n = 18). Las muestras frescas se analizaron en un espectrofotómetro de escaneo NIRSystems 6500 en modo reflectante (1100?2500 nm). El análisis de componente principal (PCA) y el análisis discriminante lineal progresivo (SLDA) se usaron para clasificar muestras de según las especie animal b... Presentar Todo |
Palabras claves : |
ANALISIS DE COMPONENTE PRINCIPAL; ANALISIS DISCRIMINANTE LINEAL PROGRESIVO; BUEY; CERDO; ESPECTROSCOPIA; INFRARROJO CERCANO; PATE. |
Thesagro : |
ALIMENTOS PROCESADOS. |
Asunto categoría : |
Q04 Composición de los alimentos |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12322/1/TCYT-A-512396-210..213-.pdf
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Marc : |
LEADER 03191naa a2200265 a 4500 001 1050733 005 2019-09-25 008 2011 bl uuuu u00u1 u #d 022 $a1947-6337 024 7 $a10.1080/19476337.2010.512396$2DOI 100 1 $aRESTAINO, E. 245 $aDiscrimination of meat patés according to the animal species by means of near infrared spectroscopy and chemometrics. [Discriminación de muestras de paté de carne según tipo de especie mediante el uso de la espectroscopia en el infrarrojo cercano y la quimiometria.]$h[electronic resource] 260 $c2011 520 $aABSTRACT. Commercial meat paté samples, comprised of 100% pork (n = 7), 100% beef (n = 5) meat, and binary mixtures (beef and pork, w/w) (n = 18) were used. Fresh samples were analysed in a scanning spectrophotometer NIRSystems 6500 in reflectance mode (1100?2500 nm). Principal component analysis (PCA) and stepwise linear discriminant analysis (SLDA) were used to classify samples according to the animal species based on their near infrared reflectance (NIR) spectra. Full cross validation was used as validation method when classification models were developed. Both beef and pork paté samples were classified correctly (100%) while binary mixture samples only achieved 72% of correct classification using SLDA technique. The results demonstrated the usefulness of NIR spectra combined with chemometrics as an objective and rapid method to classify paté samples according to meat type. Nevertheless, NIR spectroscopic methods might provide initial screening in the food chain and enable more costly methods to be used more efficiently. RESUMEN. Se utilizaron muestras de patés de carne comerciales, compuestos de 100% carne de cerdo (n = 7), 100% carne de buey (n = 5) y mezclas binarias (buey y cerdo) (n = 18). Las muestras frescas se analizaron en un espectrofotómetro de escaneo NIRSystems 6500 en modo reflectante (1100?2500 nm). El análisis de componente principal (PCA) y el análisis discriminante lineal progresivo (SLDA) se usaron para clasificar muestras de según las especie animal basándose en sus espectros NIR. La validación cruzada completa se utilizó como método de validación cuando se desarrollaron métodos de clasificación. Las muestras de ambos patés, de buey y cerdo, se clasificaron correctamente (100%), mientras las muestras de mezcla binaria sólo alcanzaron un 72% de clasificación correcta usando la técnica SLDA. Los resultados mostraron la utilidad del espectro NIR combinado con quimometria como un objetivo y método rápido para clasificar muestras de paté según el tipo de carne. Sin embargo, los métodos espectroscópicos NIR podrían proveer una revisión inicial de la cadena de comida y permitir que métodos costosos fuesen usados más eficientemente. 650 $aALIMENTOS PROCESADOS 653 $aANALISIS DE COMPONENTE PRINCIPAL 653 $aANALISIS DISCRIMINANTE LINEAL PROGRESIVO 653 $aBUEY 653 $aCERDO 653 $aESPECTROSCOPIA 653 $aINFRARROJO CERCANO 653 $aPATE 700 1 $aFASSIO, A. 700 1 $aCOZZOLINO, D. 773 $tCYTA - Journal of Food$gv. 9, n. 3, p. 210-213, 2011.
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